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lytH [2019-07-10 09:55:15]
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lytH [2019-07-10 09:55:15]

differentiation-associated peptidoglycan hydrolase involved in endospore cortex maturation
locus
BSU32340
pI
6.89
mw
39.48 kDa
protein length
326 aa Sequence Blast
gene length
981 bp Sequence Blast
function
endospore cortex maturation
product
peptidoglycan hydrolase
essential
no
synonyms
yunA,yutA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.3|Cell wall degradation/ turnover] → [category|SW 1.1.3.4|Endopeptidases]
  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.5|Cell wall/ other]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]

    • Gene

    Coordinates
    3,321,455 3,322,435

    The protein

    Catalyzed reaction/ biological activity

  • cleaves the peptide bond between L-Ala (position 1 in the peptioglycan peptide) and D-Glu (position 2) [Pubmed|18266855]

    • Protein family

  • peptidase M23B family (according to Swiss-Prot)

    • Structure

  • [PDB|3NYY] (from Ruminococcus gnavus, the C-terminal peptidase M23 domain, aa 178 ... 315, 46% identity)

    • Expression and Regulation

    Operons

    genes
    [gene|11AF08158121DEDFDDC0F64C52EFF3CF3AE30CE6|lytH]
    description
    [Pubmed|12813075]

    sigma factors

  • [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK]: sigma factor, [Pubmed|12813075], in [regulon|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK regulon]

    • regulation

  • expressed late during sporulation in the mother cell ([protein|search|SigK]) [Pubmed|12813075]
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    Biological materials

    Mutant

  • MGNA-B579 (yunA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1578 NBRP B. subtilis, Japan]
  • BKE32340 ([gene|11AF08158121DEDFDDC0F64C52EFF3CF3AE30CE6|lytH]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32340 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAAAATAATCTCCTTTCA, downstream forward: _UP4_TAACAAAAAGCCGCCTCTTC
  • BKK32340 ([gene|11AF08158121DEDFDDC0F64C52EFF3CF3AE30CE6|lytH]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32340 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAAAATAATCTCCTTTCA, downstream forward: _UP4_TAACAAAAAGCCGCCTCTTC

    • References

    Reviews

  • 18266855

    • Original publications

  • 12813075